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สรุปงานวิจัยในหัวข้อเรื่อง Biomass and biohydrogen production of unicellular green alga Chlorella vulgaris var. vulgaris TISTR 8261 in frozen food industrial wastewater
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Biomass and biohydrogen production of unicellular green alga Chlorella vulgaris var. vulgaris TISTR 8261 in frozen food industrial wastewater 

Samart Taikhao1 and Saranya Phunpruch2,3

1 Faculty of Science and Technology, Rajamangala University of Technology Suvarnabhumi, Nonthaburi 11000, Thailand

2 Department of Biology, Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand

3 Bioenergy Research Unit, Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang, Bangkok 10520, Thailand


Abstract

Green algae are able to produce H2 by electrons obtained from the photosynthetic pathway and the degradation of storage organic compounds under anaerobic condition. They can grow in various kinds of culture media containing organic and inorganic substances including essential trace minerals under photoautotrophic and photoheterotrophic conditions. To reduce the cost of nutrients for algal growth, wastewater comprising a large number of organic and inorganic compounds is used as a culture medium. This study aimed to investigate biomass and biohydrogen production by the unicellular green alga Chlorella vulgaris var. vulgaris TISTR 8261 cultivated in the frozen food industrial wastewater collected from Phra Nakhon Si Ayutthaya province. The result revealed that growth by OD750 measurement of cells cultivated in treated wastewater was obviously higher than that in untreated wastewater; however it was still lower than growth of cells in synthetic control Tris Acetate Phosphate (TAP) medium. In addition, cells showed a high ability to remove nitrate, nitrite, phosphate and sulfate from the treated wastewater. When cells were grown in various concentrations of sodium acetate (0-17.4 mM), OD750 of cultures was increased related to the higher acetate concentrations. Cells grown in treated wastewater supplemented with 17.4 mM sodium acetate exhibited the similar growth as those grown in TAP medium. The highest H2 production of 12.87 µmol H2 mg Chl a-1 was observed in cells incubated in treated wastewater supplemented with 17.4 mM sodium acetate and it was higher than that of cells incubated in TAP-N medium, supporting the possibility of using wastewater for biomass and biohydrogen production by C. vulgaris var. vulgaris TISTR 8261.


Keywords: Biomass, Biohydrogen, Food industrial wastewater, Chlorella vulgaris var. vulgaris TISTR 8261

 



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  วันที่ 11 ส.ค. 2560 เวลา 22:21:54.267

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